研究

研究室

講師:石田 洋一(イシダ ヨウイチ)

研究室

生体分子学

最終学歴

京都薬科大学大学院薬学研究科薬学専攻博士課程(2003年3月)

学位

博士(薬学) (京都薬科大学:2003年)

講義科目

生命科学実習Ⅲ(2年A、B、C組) 臨床生化学(3年C組) 卒業研究コースA実習 卒業研究II 卒業研究B

専門分野

細胞生物学 ウイルス学 プロテオミクス

研究課題

RNA代謝関連複合体の同定と機能解析 ウイルス性発がんの発症機構の解析

所属学会

日本薬学会 日本HTLV-1学会 日本分子生物学会 日本生化学会 日本プロテオーム学会

学内委員

学生厚生委員、進路就職支援委員、薬品管理システム運営委員

研究業績1

Yo-ichi Ishida*, Kiyoshi Yamashita, Hidenori Sasaki, Ichirou Takajou, Yoko Kubuki, Kazuhiro Morishita, Hirohito Tsubouchi, Akihiko Okayama. Activation of complement system in adult T-cell leukemia (ATL) occurs mainly through lectin pathway: a serum proteomic approach using mass spectrometry. Cancer Letters, 271, 167-177, 2008. *; Corresponding author

研究業績2

Yo-ichi Ishida*, Masao Yamasaki, Chizuko Yukizaki, Kazuo Nishiyama, Hirohito Tsubouchi, Akihiko Okayama, Hiroaki Kataoka. Carnosol, rosemary ingredient, induces apoptosis in adult T-cell leukemia/lymphoma cells via glutathione depletion; proteomic approach using fluorescent two-dimensional differential gel electrophoresis. Human Cell, 27, 68-77, 2014. *; Corresponding author

研究業績3

Yo-ichi Ishida, Maria Takikawa, Toshihiro Suzuki, Masami Nagahama, Yuki Ogasawara. Irreversible hyperoxidation of peroxiredoxin 2 is caused by tert-butyl hydroperoxide in human red blood cells. FEBS Open Bio, 4, 848-852, 2014.

研究業績4

Ogasawara Y.*, Ishida YI.*, Takikawa M., Funaki Y., Suzuki T., Koike S. A simple high performance liquid chromatography method for quantitatively determining the reduced form of peroxiredoxin 2 and the mass spectrometric analysis of its oxidative status. Journal of Chromatography B, Analytical Technologies in the Biomedical and Life Sciences, 997, 136-141, 2015. *; Co-first authors

研究業績5

Yuki Yoshikatsu, Yo-ichi Ishida, Haruka Sudo, Keizo Yuasa, Akihiko Tsuji, Masami Nagahama. NVL2, a nucleolar AAA-ATPase, is associated with the nuclear exosome and is involved in pre-rRNA processing. Biochemical and Biophysical Research Communications, 464, 780-786, 2015.

研究業績6

Nobuhiro Hiraishi, Yo-ichi Ishida, Masami Nagahama. AAA-ATPase NVL2 acts on MTR4-exosome complex to dissociate the nucleolar protein WDR74. Biochemical and Biophysical Research Communications, 467, 534-540, 2015.

研究業績7

Haruka Sudo, Aya Nozaki, Hideaki Uno, Yo-ichi Ishida, Masami Nagahama. Interaction properties of human TRAMP-like proteins and their role in pre-rRNA 5'ETS turnover. FEBS Letters, 590, 2963-2972, 2016.

研究業績8

Yo-ichi Ishida*, Mariko Aki, Sohta Fujiwara, Masami Nagahama, Yuki Ogasawara*. Peroxidatic cysteine residue of peroxiredoxin 2 separated from human red blood cells treated by tert-butyl hydroperoxide is hyperoxidized into sulfinic and sulfonic acids. Human Cell, Epub ahead of print. *; Co-corresponding author

研究業績9

Yo-ichi Ishida, Tasuku Kayama, Yosuke Kibune, Shoichi Nishimoto, Shin Koike, Toshihiro Suzuki, Yasue Horiuchi, Mitsuhiro Miyashita, Masanari Itokawa, Makoto Arai, Yuki Ogasawara. Identification of an argpyrimidine-modified protein in human red blood cells from schizophrenic patients: a possible biomarker for diseases involving carbonyl stress. Biochemical and Biophysical Research Communications, Epub ahead of print.

研究業績10

Nobuhiro Hiraishi*, Yo-ichi Ishida*, Haruka Sudo, Masami Nagahama. WDR74 participates in an early cleavage of the pre-rRNA processing pathway in cooperation with the nucleolar AAA-ATPase NVL2. Biochemical and Biophysical Research Communications, in press. *; Co-first authors

研究業績11

Katsutoshi Imamura, Akiko Takaya, Yo-ichi Ishida, Yayoi Fukuoka, Toshiki Taya, Ryo Nakaki, Miho Kakeda, Naoto Imamachi, Aiko Sato, Toshimichi Yamada, Rena Onoguchi-Mizutani, Gen Akizuki, Tanzina Tanu, Kazuyuki Tao, Sotaro Miyao, Yutaka Suzuki, Masami Nagahama, Tomoko Yamamoto, Torben Heick Jensen, Nobuyoshi Akimitsu. Diminished nuclear RNA decay upon Salmonella infection upregulates antibacterial noncoding RNAs. The EMBO Journal, pii: e97723, doi: 10.15252/embj.201797723, 2018.

研究業績12

Reiko Ikeda, Tomoe Ichikawa, Yu-ki Tsukiji, Kohei Kawamura, Ayano Kikuchi, Yo-ichi Ishida, Yuki Ogasawara. Identification of heparin-binding proteins on the cell surface of Cryptococcus neoformans. Medical Mycology Journal, 59E, E 47-E 52, 2018.